Chemicals and Chemistry
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Clinical Chemistry
Studies from E.J. White et al in the area of clinical chemistry described
February 12th, 2010
"Epidemiologic studies require identification or typing of microbial strains. Macrorestriction DNA mapping analyzed by pulsed-field gel electrophoresis (PFGE) is considered the current gold standard of genomic typing," scientists in the United States report. "This technique, however, is difficult to implement because it is labor-intensive and difficult to automate, it requires a long time to obtain results, and results often vary between laboratories. We used direct linear analysis (DLA), which uses a single reagent set and long fragments of microbial genomic DNA to identify various microbes. In this technique, an automated system extracts fragments exceeding 100 kb from...
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Source: Chemicals and Chemistry (2010-02-12)
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