Ecology, Environment and Conservation Business
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Scientists at Environmental Protection Agency detail research in environmental microbiology
October 3rd, 2009
According to recent research published in the journal Applied and Environmental Microbiology, "Assessment of health risk and fecal bacterial loads associated with human fecal pollution requires reliable host-specific analytical methods and a rapid quantification approach. We report the development of quantitative PCR assays for quantification of two recently described human-specific genetic markers targeting Bacteroidales-like cell surface-associated genes."
"Each assay exhibited a range of quantification from 10 to 1 x 106 copies of target DNA. For each assay, internal amplification controls were developed to detect the presence or absence of amplification...
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Source: Ecology, Environment and Conservation Business (2009-10-03)